| Recurring Mario enemies
|
| Major villains
|
| Bowser | Bowser Jr. | Cackletta | Donkey Kong | Fawful | Kamek | Kammy Koopa | King Boo | Koopalings | Shroob | Smithy Gang | Tatanga | Wario | Wart
|
| Minor villains
|
| Birdo | Chief Chilly | Donkey Kong Jr. | Eyerok | Fryguy | Goomboss | Gooper Blooper | Mouser | Petey Piranha | Raphael Raven | Tryclyde | Valentina | Waluigi
|
| Enemies
|
| Albatoss | Big Bertha | Blooper | Bob-omb | Boo | Bullet Bill | Buzzy Beetle | Cataquack | Cheep-Cheep | Chomp | Dry Bones | Fly Guy | Goomba | Hammer Bro | Koopa Troopa | Lakitu | Magikoopa | Monty Mole | Piranha Plant | Pokey | Shy Guy | Snapjaw | Snifit | Thwomp | Ukiki | Whomp | Wiggler
|
