The GENtle manual

About - FAQ - Setup - DNA - Protein - PCR and Primer Design - Sequencing - Alignments - Calculators - Virtual Gel - Image Viewer - Tools and Dialogs - Web interface - Graphs and plots - DNA map - Sequence map - Dot plot - Restriction Identifier

FAQ - frequently asked questions.

Q: This manual sucks! And I want one in my language! What can I do?
A: It's a wiki! You can edit any page and improve the manual yourself. You can even start and maintain a new translation (contact the programmer through this page).

Q: Why does GENtle try to connect to the internet all the time?
A: An internet connection is mandatory for BLAST- and ReBase-searches, as well as for the online tools.
A: At the beginning of each GENtle session, a check for possible updates takes place, which also requires an internet connection. This can be turned off in the Tools/Options menu.

Q: Why can't I perform a BLAST search for the amino acids coded by the selected DNA sequence?
Q: Why can't I extract amino acids from the selected DNA sequence?
A: A reading frame must be selected.

Q: There's a problem with multiple users using GENtle on my Windows machine.
A: You might have to allow write access to "local.db" in the GENtle directory for all users involved.
A: You can turn off the "registry settings bug" via Tools/Options/Register protocols and extensions on startup

Q: New updates download, but don't install.
A: You need write access to the GENtle program directory. Alternatively, ask your friendly administrator to download the latest version of GENtle here and install it for you. Remind him that he'll have to do this every few weeks, which might convince him to give you write access...

Q: I found a bug! And I want new functions as well! Can my favourite protease/marker/restriction enzyme be in the default installation? Where can I donate huge amounts of money for a poor programmer?
A: Add a bug report or a feature request right here in the wiki, or contact the author of GENtle through the GENtle site.

New proteases to add!


  1. Open any DNA or amino acid sequence
  2. Double-click the sequence
  3. Go to "Proteases"
  4. Click on "New protease"
  5. Enter name and sequence (one of the following)

See here for a more detailed description.