GENtle/Automatic annotation

GENtle: Tools and Dialogs

Ligation - Options - Databases - Import - Enter sequence - Sequence editor - Restriction Assistant - PCR troubleshoot dialog - Proteolysis assistant - Projects - Edit primer dialog - Find dialog - Printing - Enzyme management - Sequencing Primers - Silent Mutagenesis - Automatic annotation

The automatic annotation feature can search a database of standard vectors (included with the GENtle package), and (optionally) a user-generated database, for feature sequences that are found in the currently opened DNA sequence. Recognized features are then annotated in the current sequence.

The automatic annotation dialog.

Invoked through Edit/Auto-annotate sequence or F9, a dialog opens, offering various settings:

  • Whether or not to search the common vectors database
  • Whether or not to use a user-generated database (and, if so, which one)
  • Whether or not to reduce the number of generated features (recommended; otherwise, a lot of features are annotated)
  • Whether or not to add unrecognized open reading frames as features