Three Dimensional Electron Microscopy

About this book

This book is on three-dimensional electron microscopy (sometime referred to as 3DEM) that includes both cryo-electron microscopy and cyro-electron tomography. The goal of this book is to take all of the material on 3DEM and put it into an organized form. This organized book can then in turn be used for training new scientists in the field, teaching a course on 3DEM, or just expanding ones knowledge on the field.

Introductory Material

• What is 3DEM?
• Electron microscopes
• Particle Picking
• Fourier transforms
• CTF estimation and correction
• 2D particle alignment
• Initial model
• 3D Reconstruction
• Model validation
• Electron density interpretation

Useful Wiki Resources

• The Wikipedia article on Cryo-electron microscopy
• The Wikibook Software Tools For Molecular Microscopy
• The Wikibook chapter Structural Biochemistry/Proteins/Cryo-Electron Microscopy

Useful Off-site Resources

• 3-dimensional electron microscopy (3DEM) mailing list