Proteomics/Protein Separations- Electrophoresis/Timelines of Electrophoresis
Timeline of Electrophoresis edit
‘‘‘1930s: Use of sucrose for gel electrophoresis.
‘‘‘1937: The official launching of Tiselius' moving boundary electrophoresis (MBE).
‘‘‘1939: Zone Electrophoresis
‘‘‘1950: Rich in introducing novel methodologies in zone electrophoresis (ZE)
‘‘‘1950: ‘‘‘Agar Gel electrophoresis
‘‘‘1955: Introduction of starch gels
‘‘‘1957: Cellulose acetate Electrophoresis (CAc) by Kohn
‘‘‘1959: Introduction of acrylamide gels by Raymond and Winstraub
‘‘‘1961: Electrophoresis in silica gel by Honnegar
‘‘‘1961: IsoElectricFocusing by Svensson
‘‘‘1964: Electrophoresis in thin layer of sephadex beads by Fasella et.al
‘‘‘1964: Disc gel electrophoresis Ornstein and Davis
‘‘‘1969: Introduction of denaturing agents especially SDS separation of protein subunit by Beber and Osborn
‘‘‘1970: T4 phage proteins were separated using stacking gel and SDS by Laemmli
‘‘‘1975: 2 Dimensional gels: Isoelectric focusing and SDS gel electrophoresis by OFarrell.
‘‘‘1977: Sequencing gels
‘‘‘1970s: agars gels
‘‘‘1980s: Immobilized pH gradients
‘‘‘1983: ‘‘‘Pulsed field gel electrophoresis
‘‘‘1983: ‘‘‘Capillary electrophoresis
References edit
- Electrophoresis: the march of pennies, the march of dimes. Righetti PG.
- http://www.csupomona.edu/~nebuckley/Courses/Bio451/Lectures/Bio451Lecture4.htm