Molecular Cloning

Contents

1. Plasmid
   • Plasmid extraction
   • Preparation of competent cell (E.Coli)
2. Extraction of DNA
   • Southern hybridization
3. PCR
4. Checking of DNA on PAGE
   • Extraction of Protein
5. SDS-PAGE (Polyacrylamide gel electrophoresis)
   • western blot