Last modified on 3 June 2014, at 10:19

Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Bottom-up proteomics


Bottom-up versus top-down proteomics

Bottom-up proteomics is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry.[1][2] The proteins may first be purified by a method such as gel electrophoresis resulting in one or a few proteins in each proteolytic digest. Alternatively, the crude protein extract is digested directly, followed by one or more dimensions of separation of the peptides by liquid chromatography coupled to mass spectrometry, a technique known as shotgun proteomics.[3][4] By comparing the masses of the proteolytic peptides or their tandem mass spectra with those predicted from a sequence database, peptides can be identified and multiple peptide identifications assembled into a protein identification.

ReferencesEdit

  1. Aebersold R, Mann M (March 2003). "Mass spectrometry-based proteomics". Nature 422 (6928): 198–207. doi:10.1038/nature01511. PMID 12634793. 
  2. Chait BT (2006). "Chemistry. Mass spectrometry: bottom-up or top-down?". Science 314 (5796): 65–6. doi:10.1126/science.1133987. PMID 17023639. 
  3. Washburn MP, Wolters D, Yates JR (2001). "Large-scale analysis of the yeast proteome by multidimensional protein identification technology". Nat. Biotechnol. 19 (3): 242–247. doi:10.1038/85686. PMID 11231557. 
  4. Wolters DA, Washburn MP, Yates JR (2001). "An automated multidimensional protein identification technology for shotgun proteomics". Anal. Chem. 73 (23): 5683–5690. doi:10.1021/ac010617e. PMID 11774908.